General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen δ Opioid Receptor Membrane Preparation, 1 vial. (HTS100M)
The ChemiScreen δ Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRD1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter CHO-K1 LXRα Nuclear Translocation Cell Line, 2 vials. (93-0215C2)
The PathHunter LXRα Nuclear Translocation cell line is a stable, engineered cell line for use in studying drug candidates affecting NHR activation and nuclear translocation. This cell line enables assessment of ligand (e.g. oxysterols) based NHR activation via detection of resulting LXRα nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged LXRα NHR and nuclear localized EA. Upon activation of LXRα, induced nuclear translocation leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter CHO-K1 CHRM4 β-Arrestin Cell Line, 2 vials. (93-0349C2)
The PathHunter CHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM4 and EA-tagged β-Arrestin-2. Activation of CHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 CRTH2 Gi Cell Line, 2 vials. (95-0072C2)
The cAMP Hunter CRTH2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin D2) based activation of CRTH2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CRTH2, a naturally Gi/Go coupled GPCR. Upon activation of CRTH2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section - Human Gallbladder Tumor: Adenocarcinoma, Poorly Differentiated, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx PathHunter P2RY12 Total Internalization U2OS Cell Line, 2 vials. (93-0952C3)
The PathHunter P2RY12 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of P2RY12 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged P2RY12 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the P2RY12 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 ADRB1 Gs Cell Line, 2 vials. (95-0113C2)
The cAMP Hunter ADRB1 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. isoproterenol) based activation of ADRB1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB1, a naturally Gs coupled GPCR. Upon activation of ADRB1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Pasteurella multocida; subsp. multocida
Pasteurella multocida subsp. multocida strain NCTC 10325 is a bacterium that was isolated from a bovine in the United States.
Eurofins Discoverx PathHunter CHO-K1 mGPR91 (SUCNR1) β-Arrestin Cell Pool, 2 vials. (93-0589P2)
The PathHunter mGPR91 (SUCNR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. succinate) based activation of mGPR91 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGPR91 and EA-tagged β-Arrestin-2. Activation of mGPR91 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable cell pool is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section - Human Bone Tumor: Myeloma, Plasmacytic, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Paraffin Tissue Section - Parkinson's Disease: Brain: Precentral Gyrus, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from single human diseased sample. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Frozen Tissue Section - Arrhythmia, infarct: Heart Ventricle, left, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx PathHunter U2OS TGFBR1A/ACVR2A Dimerization Cell Line, 2 vials. (93-1012C3)
The PathHunter TGFBR1A/ACVR2A Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. GDF-11) induced dimerization of TGFBR1A/ACVR2A receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1A target protein fused to PK alongside ACVR2A target protein fused to EA. Upon ligand-induced dimerization of TGFBR1A/ACVR2A, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
ATCC Staphylococcus aureus; subsp. aureus; Strain 252
Staphylococcus aureus subsp. aureus strain MRSA252 is a hospital-acquired methicillin-resistant strain (MRSA) isolated in the United Kingdom. This whole-genome sequenced bacterial strain has applications in antimicrobial resistance research and drug development.